Invitrogen CyQUANT MTT Cell Viability Assay

CytoSelect™ MTT Cell Proliferation Assay mtt The MTT assay is a quantitative and sensitive detection of cell proliferation as it measures the growth rate of cells by virtue of a linear relationship between mtt Steps · 1 Discard media from cell cultures · 2 Add 50 µL of serum-free media and 50 µL of MTT solution into each well · 3 Incubate the

mtt The reduced MTT forms insoluble formazan which accumulates both inside of and on the surface of cells Generation of formazan reflects viable cell density