DEAE CELLULOSE

DEAE Sepharose is a fast flow, weak anion exchanger suitable for the initial purification of ribonucleoproteins from cell crude extracts

This method is best performed using a batch method The DEAE beads can be kept in a disposable syringe containing a polypropylene frit, a glass

deae Theoretically, HP-NAP is negatively charged above its pI However, the recombinant HP-NAP obtained from the unbound fractions from DEAE ion-exchange

deae If the antibody solution is adjusted to a basic pH of 8–, then IgG binds to the DEAE resin After washing the column, the antibody is eluted by adding a